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    Molecular detection of Toxoplasma gondii in ready-to-eat salad mixes: multi-country survey using a validated and harmonised standard operating procedure, Europe, 2021 to 2022

    Eurosurveillance

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    Data
    2025
    Autor
    Calero-Bernal, Rafael
    Betson, Martha
    Slana, Iva
    Bartosova, Barbora
    Marucci, Gianluca
    Possenti, Alessia
    Álvarez-García, Gema
    Bier, Nadja
    Mayer-Scholl, Anne
    Berg, Rebecca P.
    Chaudhry, Umer
    López-Ureña, Nadia M.
    Piotrowska, Weronika
    Sroka, Jacek
    Johannessen, Gro S.
    Davidson, Rebecca
    Dámek, Filip
    Blaga, Radu
    Thoumire, Sandra
    Zalewská, Barbora
    Waap, Helga C.
    Jokelainen, Pikka
    Lalle, Marco
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    Streszczenie
    Background Most Toxoplasma gondii infections in humans are considered foodborne, but the relative importance of the various routes of infection is largely unknown. Consumption of green produce contaminated with T. gondii oocysts has been identified as a possible source. Aim We aimed to estimate the occurrence and prevalence of T. gondii oocysts in commercially available ready-to-eat (RTE) salad mixes in 10 European countries. Methods A real-time PCR-based method for oocyst detection was developed and optimised by two laboratories and validated in an interlaboratory test. This detection method and a harmonised sampling strategy were applied in a multi-country study. Multivariable logistic regression was used to investigate risk factors for oocyst contamination of RTE salad. Results The real-time PCR method had a detection limit of 10 oocysts per 30 g of salad. We collected 3,329 RTE salad samples (baby leaf and cut leaf mixes) from October 2021 to September 2022. The prevalence of T. gondii oocyst contamination was 4.1% (95% confidence interval (CI): 3.4–4.8%; n = 3,293). In multivariable regression analysis, winter season, sampling and packaging of salad in Northern Europe and production of salad in Western Europe were associated with detection of T. gondii, with no statistically significant differences between salad types. Conclusion We estimated the prevalence of T. gondii oocysts in RTE leafy green salads using a validated and standardised procedure to assess the potential risk for human infection; highlighting the need to address this risk at each critical point of the salad production chain.
    URI
    https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2025.30.22.2400594#html_fulltext
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