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Carbohydrate Research

dc.contributor.authorKurzylewska, Maria
dc.contributor.authorBomba, Arkadiusz
dc.contributor.authorDworaczek, Katarzyna
dc.contributor.authorPękala-Safińska, Agnieszka
dc.contributor.authorTurska-Szewczuk, Anna
dc.date.accessioned2023-05-12T12:27:25Z
dc.date.available2023-05-12T12:27:25Z
dc.date.issued2023
dc.identifierhttps://dspace.piwet.pulawy.pl/xmlui/handle/123456789/514
dc.identifier.issn0008-6215
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S000862152300071X
dc.description.abstractAeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup. The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy. The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established. →2)[α-D-Fucp3NRHb-(1→3)]-α-L Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases. The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.
dc.language.isoEN
dc.publisherELSEVIER SCI LTD
dc.subjectAeromonas
dc.subjectO-antigen
dc.subjectO-antigen gene cluster
dc.subjectWzx/Wzy pathway
dc.subjectFucp3N
dc.titleStructure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup
dcterms.bibliographicCitation2023 Vol. 528, 108809
dcterms.titleCarbohydrate Research
dc.identifier.doi10.1016/j.carres.2023.108809


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