Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy

    • Zaloguj
    Zobacz pozycję 
    •   Strona główna Repozytorium
    • PIWet - PIB
    • Publikacje
    • Zobacz pozycję
    •   Strona główna Repozytorium
    • PIWet - PIB
    • Publikacje
    • Zobacz pozycję
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Diagnosis and phylogenetic analysis of bovine viral diarrhea virus in cattle (Bos taurus) and buffaloes (Bubalus bubalis) from the Amazon region and Southeast Brazil

    Pesquisa Veterinaria Brasilera

    Thumbnail
    Oglądaj/Open
    document.pdf (787.5KB)
    Data
    2022
    Autor
    Assunção, Stella
    Antos, Aleksandra
    Barbosa, José
    Reis, Jenner
    Larska, Magdalena
    Oliveira, Cairo
    Metadane
    Pokaż pełny rekord
    Streszczenie
    Bovine viral diarrhea virus (BVDV) is a highly infectious pathogen that affects bovinesworldwide leading to great economic impact. Although Brazil has the largest commercial cattlepopulation throughout the world and an increasing buffalo breeding industry, the country hasno control or eradication program for BVDV. In this perspective, the aim of this study was toevaluate the occurrence of BVDV in cattle and buffaloes from two Brazilian states. Four differentELISA tests were performed and confirmed by virus neutralization testing (VNT). The presenceof BVDV antibodies in the serum or plasma from 77 cattle from six herds (ELISA-1 and ELISA-4)and from 89 buffaloes from three herds (ELISA-1 through ELISA-4) was detected. Extractionof viral RNA was performed from the serum or plasma samples for the detection of BVDV byRT-PCR analysis. Amplified nucleotide sequences were used to construct a phylogenetic tree.In cattle, ELISA-1 detected 49.4% of seropositive animals, while ELISA-4 detected 37.7%. Inbuffaloes, ELISA-1 failed to detect any seropositive animals, while ELISA-2 and ELISA-3 detected20.2% of seropositive animals, and ELISA-4 detected 21.3%. Eight of the nine herds tested hadseropositive animals. The rate of PCR positive animals was 6.5% in cattle and 9% in buffaloes.Subtype 1d was found in cattle, and subtypes 1d and 1f were found in buffaloes. This is the first-time subtype 1f has been reported in Brazil. The absence of a control and eradication programseems to be favoring the spread of BVDV in the Brazilian herds. In addition, the improvementof diagnostic strategies for BVDV in buffaloes are required.
    URI
    https://www.scielo.br/j/pvb/a/8Fvb4hyTpj5mS5VnMsd74NP/
    Zbiory
    • Publikacje [635]

    DSpace software copyright © 2002-2016  DuraSpace
    Kontakt z nami | Wyślij uwagi
    Theme by 
    Atmire NV
     

     

    Przeglądaj

    Całe RepozytoriumZbiory i kolekcjeDaty wydaniaAutorzyTytułyTematyTa kolekcjaDaty wydaniaAutorzyTytułyTematy

    Moje konto

    Zaloguj

    Statystyki

    Przejrzyj statystyki użycia

    DSpace software copyright © 2002-2016  DuraSpace
    Kontakt z nami | Wyślij uwagi
    Theme by 
    Atmire NV