The first evidence on the occurrence of bisphenol analogues in the aqueous humor of patients undergoing cataract surger
Journal of Clinical Medicine
dc.contributor.author | Flieger Jolanta | |
dc.contributor.author | Śniegocki Tomasz | |
dc.contributor.author | Dolar-Szczasny Joanna | |
dc.contributor.author | Załuska Wojciech | |
dc.contributor.author | Rejdak Robert | |
dc.date.accessioned | 2022-11-03T11:47:20Z | |
dc.date.available | 2022-11-03T11:47:20Z | |
dc.date.issued | 2022 | |
dc.identifier | https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/380 | |
dc.identifier.issn | 2077-0383 | |
dc.identifier.uri | https://www.mdpi.com/2077-0383/11/21/6402 | |
dc.description.abstract | Human exposure to BPs is inevitable mostly due to contaminated food. In this prelimi-nary study, for the first time, the presence of bisphenols (BPs) in aqueous humor (AH) collectedfrom 44 patients undergoing cataract surgery was investigated. The measurements were per-formed using a sensitive ultra-high performance liquid chromatography-tandem mass spectrome-try (UHPLC–MS/MS). Chromatographic separation was achieved using a reverse-phase columnand a gradient elution mode. Multiple reaction monitoring (MRM) was used. The method wasvalidated for bisphenol A (BPA) and bisphenol F (BPF). The limits of quantification (LOQs) of bothinvestigated analytes were 0.25 ng mL−1. The method was linear in the range of 0.25–20.0 ng mL−1with correlation coefficients (R2) higher than 0.98. Recovery of analytes was in the range of 99.9 to104.3% and intra-assay and inter-assay precision expressed by relative standard deviations (RSD%)were less than 5%. BPA was detected in 12 AH samples with mean concentrations of 1.41 ng mL−1.BPF was not detected at all. Furthermore, two structural isomers termed BPA-1, and BPA-2 wereidentified, for the first time, in 40.9% of the AH samples, with almost twice higher mean concen-trations of 2.15 ng mL−1, and 2.25 ng mL−1, respectively. The total content of BPs were higher inpatients with coexisting ocular pathologies such as glaucoma, age-related macular degeneration(AMD), and diabetes in comparison to cataracts alone. However, the difference between thesegroups did not reach statistical significance (p > 0.05). Performed investigations indicate the needfor further research on a larger population with the aim of knowing the consequences of BPs’ ac-cumulation in AH for visual function. | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.subject | bisphenols | |
dc.subject | bisphenol A | |
dc.subject | bisphenol F | |
dc.subject | bisphenol A isomers | |
dc.subject | aqueous humor | |
dc.subject | ultrahigh-pressure liquid chromatography (UHPLC) | |
dc.title | The first evidence on the occurrence of bisphenol analogues in the aqueous humor of patients undergoing cataract surger | |
dcterms.bibliographicCitation | 2022 vol. 11 s. 6402 | |
dcterms.title | Journal of Clinical Medicine | |
dc.identifier.doi | 10.3390/jcm11216402 |
Pliki tej pozycji
Pozycja umieszczona jest w następujących kolekcjach
-
Publikacje [580]
artykuły z czasopism