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International Journal of Molecular Sciences

dc.contributor.authorCapo, Alessandro
dc.contributor.authorCalabrese, Alessia
dc.contributor.authorFrant, Maciej
dc.contributor.authorWalczak, Marek
dc.contributor.authorSzczotka-Bochniarz, Anna
dc.contributor.authorManessis, Georgios
dc.contributor.authorBossis, Ioannis
dc.contributor.authorStaiano, Maria
dc.contributor.authorD’Auria, Sabato
dc.contributor.authorVarriale, Antonio
dc.date.accessioned2022-07-12T11:38:18Z
dc.date.available2022-07-12T11:38:18Z
dc.date.issued2022
dc.identifierhttps://dspace.piwet.pulawy.pl/xmlui/handle/123456789/304
dc.identifier.issn1422-0067
dc.identifier.urihttps://www.mdpi.com/1422-0067/23/13/7463
dc.description.abstractAfrican swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 103 HAD50/mL.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectAfrican swine fever virus (ASFV)en_US
dc.subjectdetectionen_US
dc.subjectantibodyen_US
dc.subjectindirect ELISA testen_US
dc.subjectsurface plasmon resonance (SPR)en_US
dc.titleSPR-Based Detection of ASF Virus in Cellsen_US
dc.typeArticleen_US
dcterms.bibliographicCitation2022 vol. 23 nr 13 s. 7463
dcterms.titleInternational Journal of Molecular Sciences
dc.identifier.doihttps://doi.org/10.3390/ijms23137463


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