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Letters in Applied Microbiology

dc.contributor.authorPerrin-Guyomard, Agnes
dc.contributor.authorGranier, Sophie A.
dc.contributor.authorSlettemeas, Jannice Schau
dc.contributor.authorAnjum, Muna
dc.contributor.authorRandall, Luke
dc.contributor.authorAbuOun, Manal
dc.contributor.authorNatalie, Pauly
dc.contributor.authorIrrgang, Alexandra
dc.contributor.authorHammerl, Jens Andre
dc.contributor.authorKjeldgaard, Jette Sejer
dc.contributor.authorHammerum, Anette
dc.contributor.authorFranco, Alessia
dc.contributor.authorSkarżyńska, Magdalena
dc.contributor.authorKamińska, Ewelina
dc.contributor.authorWasyl, Dariusz
dc.contributor.authorDierikx, Cindy
dc.contributor.authorBorjesson, Stefan
dc.contributor.authorGeurts, Yvon
dc.contributor.authorHaenni, Marisa
dc.contributor.authorVeldman, Kees
dc.description.abstractThis study was conducted to evaluate the performance of a screening protocol to detect and isolate mcr-positive Escherichia coli and Salmonella spp. from animal caecal content and meat samples. We used a multicentre approach involving 12 laboratories from nine European countries. All participants applied the same methodology combining a multiplex PCR performed on DNA extracted from a pre-enrichment step, followed by a selective culture step on three commercially available chromogenic agar plates. The test panel was composed of two negative samples and four samples artificially contaminated with E. coli and Salmonella spp. respectively harbouring mcr- 1 or mcr-3 and mcr-4 or mcr-5 genes. PCR screening resulted in a specificity of 100% and a sensitivity of 83%. Sensitivity of each agar medium to detect mcr-positive colistin-resistant E. coli or Salmonella spp. strains was 86% for CHROMIDâ Colistin R, 75% for CHROMagarTM COL-APSE and 70% for COLISTIGRAM. This combined method was effective to detect and isolate most of the E. coli or Salmonella spp. strains harbouring different mcr genes from food-producing animals and food products and might thus be used as a harmonized protocol for the screening of mcr genes in food-producing animals and food products in Europe.en_US
dc.subjectColistin resistanceen_US
dc.subjectE. colien_US
dc.titleMulticentre evaluation of a selective isolation protocol for detection of mcr ‐positive E. coli and Salmonella spp. in food‐producing animals and meaten_US
dcterms.titleLetters in Applied Microbiology

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