Pokaż uproszczony rekord


dc.contributor.authorOlech, Monika
dc.contributor.authorBomba, Arkadiusz
dc.contributor.authorKuźmak, Jacek
dc.description.abstractSmall ruminant lentiviruses (SRLVs) exist as populations of closely related genetic variants, known as quasispecies, within an individual host. The privileged way of SRLVs transmission in goats is through the ingestion of colostrum and milk of infected does. Thus, characterization of SRLV variants transmitted through the milk, including milk epithelial cells (MEC), may provide useful information about the transmission and evolution of SRLVs. Therefore, the aim of this study was to detect SRLVs in peripheral blood leukocytes (PBLs) and milk epithelial cells of goats naturally infected with SRLVs and perform single nucleotide variations analysis to characterize the extent of genetic heterogeneity of detected SRLVs through comparison of their gag gene sequences. Blood and milk samples from 24 seropositive goats were tested in this study. The double immunolabeling against p28 and cytokeratin demonstrated that milk epithelial cells originated from naturally infected goats were infected by SRLVs. Moreover, PCR confirmed the presence of the integrated SRLVs proviral genome indicating that MECs may have a role as a reservoir of SRLVs and can transmit the virus through milk. The blood and MEC derived sequences from 7 goats were successfully sequenced using NGS and revealed that these sequences were genetically similar. The MEC and blood-derived sequences contained from 3 to 30 (mean, 10.8) and from 1 to 10 (mean, 5.4) unique SNVs, respectively. In five out of seven goats, SNVs occurred more frequent in MEC derived sequences. Non-synonymous SNVs were found in both, PBLs and MEC-derived sequences of analyzed goats and their total number differed between animals. The results of this study add to our understanding of SRLVs genomic variability. Our data provides evidence for the existence of SRLVs quasispecies and to our knowledge, this is the first study that showed quasispecies composition and minority variants of S! RLVs present milk epithelial cells.en_US
dc.subjectnext-generation sequencing (NGS)en_US
dc.subjectsingle nucleotide variations (SNVs)en_US
dc.subjectmilk epithelial cells (MEC)en_US
dc.subjectsmall ruminant lentiviruses (SRLVs)en_US
dc.subjectsmall ruminant lentiviruses (SRLVs); milk epithelial cells (MEC); single nucleotide variations (SNVs); next-generation sequencing (NGS)en_US
dc.titleQuasispecies composition of small ruminant lentiviruses found in blood leukocytes and milk epithelial cellsen_US
dcterms.bibliographicCitation2021 vol. 13 nr 12 s. 2497

Pliki tej pozycji


Pozycja umieszczona jest w następujących kolekcjach

Pokaż uproszczony rekord