Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy

    • Zaloguj
    Zobacz pozycję 
    •   Strona główna Repozytorium
    • PIWet - PIB
    • Publikacje
    • Zobacz pozycję
    •   Strona główna Repozytorium
    • PIWet - PIB
    • Publikacje
    • Zobacz pozycję
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

    PLoS One

    Thumbnail
    Oglądaj/Open
    journal.pone.0258318.pdf (2.417MB)
    Data
    2021
    Autor
    Antas, Marta
    Olech, Monika
    Szczotka-Bochniarz, Anna
    Metadane
    Pokaż pełny rekord
    Streszczenie
    Porcine epidemic diarrhoea (PED) is a highly contagious enteric viral disease of pigs with a high morbidity and mortality rate, which ultimately results in huge economic losses in the pig production sector. The etiological agent of this disease is the porcine epidemic diarrhoea virus (PEDV) which is an enveloped, positive single-stranded RNA virus. The aim of this study was to perform molecular characterization of PEDV to identify the strains circulating in Poland. In this study, 662 faecal samples from 2015 to 2021 were tested with reverse transcription quantitative real-time PCR (RT-qPCR) and the results showed that 3.8% of the tested samples revealed a positive result for PEDV. A phylogenetic analysis of the complete genome and complete S gene sequences showed that Polish PEDV strains belonged to the G1b (S-INDEL) subgroup and were closely related to the European PEDV strains isolated from 2014 to 2019. Furthermore, RDP4 analysis revealed that the Polish PEDV strains harboured a recombinant fragment of ~400 nt in the 5’ end of S gene with PEDV and swine enteric coronavirus (SeCoV) being the major and minor parents, respectively. Antigenic analysis showed that the aa sequences of neutralizing epitopes were conserved among the Polish PEDV strains. Only one strain, #0100/5P, had a unique substitution in the COE epitope. However, Polish PEDV strains showed several substitutions, especially in the COE antigen, as compared to the classical strain CV777. To the best of our knowledge, this is the first report concerning the molecular characterization of porcine epidemic diarrhoea virus strains, as well as the first phylogenetic analysis for PEDV in Poland.
    URI
    https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258318
    Zbiory
    • Publikacje [640]

    DSpace software copyright © 2002-2016  DuraSpace
    Kontakt z nami | Wyślij uwagi
    Theme by 
    Atmire NV
     

     

    Przeglądaj

    Całe RepozytoriumZbiory i kolekcjeDaty wydaniaAutorzyTytułyTematyTa kolekcjaDaty wydaniaAutorzyTytułyTematy

    Moje konto

    Zaloguj

    Statystyki

    Przejrzyj statystyki użycia

    DSpace software copyright © 2002-2016  DuraSpace
    Kontakt z nami | Wyślij uwagi
    Theme by 
    Atmire NV