Przeglądaj według autora "Kuźmak, Jacek"
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An outbred calf model for determining innate immune sensing and evolutionary trajectories of a cell culture-adapted bovine foamy virus variant
Materniak-Kornas, Magdalena; Kubiś, Piotr; Sell, Bartosz; Georgios, Pougialis; Martin, Löchelt; Kuźmak, Jacek (MDPI, 2023)Bovine foamy virus (BFVbta) displays a very high degree of cell-associated replication which is unprecedented even among the other known foamy viruses. Interestingly, recent studies have shown that it can in fact adapt in ... -
Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses
Olech, Monika; Kuźmak, Jacek (National Veterinary Research Institute in Pulawy; Poland, 2024)Introduction: Small ruminant lentiviruses (SRLV) cause multisystemic, degenerative and chronic disease in sheep and goats.There are five genotypes (A, B, C, D and E), of which A and B are the most widespread. The purpose ... -
Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus.
Szczotka, Maria; Kuźmak, Jacek (National Veterinary Research Institute in Pulawy; Poland, 2024)AbstractIntroduction: Bovine leukaemia virus (BLV) is a Deltaretrovirus responsible for enzootic bovine leukosis, the most common neoplastic disease of cattle. It deregulates the immune system, favouring secondary infections ... -
Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6)
Kubiś, Piotr; Kuźmak, Jacek (National Veterinary Research Institute in Pulawy; Poland, 2023)Introduction: Bovine herpesvirus 6 (BoHV6) belongs to the Herpesviridae family, Gammaherpesvirinae subfamily and Macavirus genus. It is common in cattle, but was also detected in American bison (Bison bison) and water ... -
Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis
Szczotka, Maria; Kuźmak, Jacek (National Veterinary Research Institute in Pulawy; Poland, 2022)Bovine leukaemia virus (BLV) is the retroviral causative agent of enzootic bovine leukosis, the most common neoplastic disease of cattle and a serious problem worldwide. Its diagnosis is commonly ... -
Genetic Diversity of the LTR Region of Polish SRLVs and Its Impact on the Transcriptional Activity of Viral Promoters
Olech, Monika; Kuźmak, Jacek (MDPI, 2023)A long terminal repeat (LTR) plays an indispensable role in small ruminant lentivirus (SRLV) gene expression. In this study, we present the LTR sequence of Polish SRLVs representing different subtypes, and analyzed their ... -
Genetic variability of Bovine Leukemia Virus: evidence of dual infection, recombination and Quasi-Species
Pluta, Aneta; Rola-Łuszczak, Marzena; Hoffmann, Federico; Donnik, Irina; Petropavlovskiy, Maxim; Kuźmak, Jacek (MDPI, 2024)AbstractWe have characterized the intrahost genetic variation in the bovine leukemia virus (BLV) by examining 16 BLV isolates originating from the Western Siberia–Tyumen and South Ural–Chelyabinsk regions of Russia. Our ... -
Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan
Sultanov, Akhmetzhan; Rola-Łuszczak, Marzena; Mamanova, Saltanat; Ryło, Anna; Osiński, Zbigniew; Saduakassova, Meruyert A.; Bashenova, Elvira; Kuźmak, Jacek (MDPI, 2022)Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leukosis (EBL) and has worldwide distribution. Infections with BLV have been reported in cattle from Kazakhstan but the virus has not yet been thoroughly ... -
Molecular Characterization of Small Ruminant Lentiviruses in Polish Mixed Flocks Supports Evidence of Cross Species Transmission, Dual Infection, a Recombination Event, and Reveals the Existence of New Subtypes within Group A
Olech, Monika; Kuźmak, Jacek (MDPI, 2021)Small ruminant lentiviruses (SRLVs) are a group of highly divergent viruses responsible for global infection in sheep and goats. In a previous study we showed that SRLV strains found in mixed flocks in Poland belonged to ... -
Molecular Characterization of Small Ruminant Lentiviruses Isolated from Polish Goats with Arthritis
Olech, Monika; Kycko, Anna; Kuźmak, Jacek (MDPI, 2022)Previous studies revealed that the small ruminant lentivirus (SRLV) population in Poland is highly heterogeneous. All SRLVs detected from Polish sheep and goats so far have belonged to subtypes B1, B2, A1, A5, A12, A13, ... -
Molecular Characterization of the env Gene of Bovine Leukemia Virus in Cattle from Pakistan with NGS-Based Evidence of Virus Heterogeneity
Rola-Łuszczak, Marzena; Sakhawat, Ali; Pluta, Aneta; Ryło, Anna; Bomba, Arkadiusz; Bibi, Nazia; Kuźmak, Jacek (2021)Characterization of the global genetic diversity of the bovine leukemia virus (BLV) is an ongoing international research effort. Up to now BLV sequences have been classified into eleven distinct genotypes. Although BLV ... -
Quasispecies composition of small ruminant lentiviruses found in blood leukocytes and milk epithelial cells
Olech, Monika; Bomba, Arkadiusz; Kuźmak, Jacek (MDPI, 2021)Small ruminant lentiviruses (SRLVs) exist as populations of closely related genetic variants, known as quasispecies, within an individual host. The privileged way of SRLVs transmission in goats is through the ingestion of ... -
Seroprevalence of feline foamy virus in domestic cats in Poland
Materniak-Kornas, Magdalena; Frymus, Tadeusz; Löchelt, Martin; Kuźmak, Jacek (National Veterinary Research Institute in Pulawy, 2021)Introduction: Feline foamy virus (FFVfca) is widespread and its prevalence in naturally infected domestic cats ranges between 30% and 80% worldwide. The infection is persistent, with a sustained antibody response in ... -
Transcriptome Analysis for Genes Associated with Small Ruminant Lentiviruses Infection in Goats of Carpathian Breed
Olech, Monika; Ropka-Molik, Katarzyna; Szmatoła, Tomasz; Piórkowska, Katarzyna; Kuźmak, Jacek (MDPI, 2021)Small ruminant lentiviruses (SRLV) are economically important viral pathogens of sheep and goats. SRLV infection may interfere in the innate and adaptive immunity of the host, and genes associated with resistance or ... -
Transcriptome analysis of bovine macrophages (BoMac) cells after infection with bovine immunodeficiency virus
Rola-Łuszczak, Marzena; Materniak-Kornas, Magdalena; Kubiś, Piotr; Pluta, Aneta; Smagacz, Marlena; Kuźmak, Jacek (National Veterinary Research Institute in Pulawy; Poland, 2022)Introduction: Bovine immunodeficiency virus (BIV) is found worldwide in cattle under natural conditions. However, the effect of BIV infection on immune functions has not been fully characterized. Material and Methods: ... -
Transcriptomic Analysis of MDBK Cells Infected with Cytopathic and Non-Cytopathic Strains of Bovine Viral Diarrhea Virus (BVDV)
Mirosław, Paweł; Rola-Łuszczak, Marzena; Kuźmak, Jacek; Polak, Mirosław P. (MDPI, 2022)Bovine viral diarrhea virus (BVDV) belongs to the Flaviviridae family and the Pestivirus genus. Infection with BVDV causes a disease with a wide spectrum of clinical symptoms, most often mild, although infections with this ...