https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/6 artykuły z czasopism Thu, 23 Jul 2026 16:48:29 GMT 2026-07-23T16:48:29Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/965 Abequose-containing O-antigen polysaccharide from Aeromonas sobria K221 (serogroup PGO1): structure, O-antigen gene cluster correlation, and Abe-transferase prediction Anna Turska-Szewczuk, Anna; Dworaczek, Katarzyna; Bomba, Arkadiusz; Kubaczyńska, Maria; Drzewiecka, Dominika; Iwan, Ewelina; Kamińska, Ewelina; Pękala-Safińska, Agnieszka Aeromonas species are important opportunistic pathogens in freshwater aquaculture, including Polish fish farms, where outbreak-associated isolates have been assigned to local provisional serogroups. Among these, PGO1 is frequently detected, but the structural and genetic diversity of the corresponding O-specific polysaccharides remains poorly understood. Here, we characterized Aeromonas sobria strain K221, a PGO1 isolate recovered from common carp during an outbreak of motile Aeromonas infection/septicaemia. The O-specific polysaccharide (OPS), isolated from LPS, was analysed by chemical methods and 1H/13C NMR spectroscopy, and its O-repeating unit was identified as a linear pentasaccharide containing β-GlcpNAc, 2-O-acetylated α-Rhap, and α-Abep resi- dues. Bioinformatic analysis of the O-antigen gene cluster (OGC) revealed gene content consistent with the OPS structure and supported functional assignment of the biosynthesis locus, including a putative α-1,3-CDP-abe- quosyltransferase. Both the OPS and its OGC differed from those of other recently characterized PGO1 strains, indicating that K221 represents a distinct variant within this serogroup. The occurrence of 2-substituted abe- quose, not previously reported in Aeromonas O-polysaccharides or as a 2-substituted residue in bacterial O- polysaccharides, highlights an unusual structure–biosynthesis relationship. These findings reveal greater struc- tural and genetic diversity within the Aeromonas PGO1 serogroup than previously recognized and expand current knowledge of bacterial O-specific carbohydrate polymers. Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/965 2026-01-01T00:00:00Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/964 MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV Gata-de-Benito, Julia; Walczak, Marek; Liu, Lihong; Vigara-Astillero, Gonzalo; Szymankiewicz, Krzesimir; Kochanowski, Maciej; Żmudzki, Jacek; Revilla, Yolanda; Pérez-Núñez, Daniel African swine fever virus (ASFV) encodes numerous multigene family (MGF) proteins whose individual contributions to virulence and immune modulation remain poorly understood. Here, we characterize the role of MGF110-2L in shaping innate immunity and disease outcome in vivo. Deletion of MGF110-2L from the virulent Arm/07/CBM/c2 isolate markedly attenuated clinical disease and conferred complete protection against homologous challenge. In macrophages, the MGF110-2L-deficient recombinant induced enhanced type I interferon (IFN) responses, consistent with a role for MGF110-2L in antagonizing antiviral signaling. However, transcriptomic profiling of peripheral blood mononuclear cells revealed that the absence of MGF110-2L prevents the excessive IFN and inflammatory hyperactivation triggered by the parental virus in vivo, establishing a controlled antiviral program associated with reduced pathology. These findings identify MGF110-2L as a functional virulence factor that drives dysregulated innate immune responses during ASFV infection. Our work advances the molecular understanding of ASFV-host interactions and highlights the contribution of MGF110-2L protein to immune evasion and pathogenesis. IMPORTANCEFinding safe and effective vaccines is essential to impair the spread of African swine fever virus (ASFV), responsible for the largest animal pandemic. Identifying ASFV virulence factors and understanding the mechanisms of pathogenesis and protection are crucial. By using RNAseq from in vivo peripheral blood mononuclear cells, together with immunological studies, we showed that MGF110-2L regulates type I IFN production and cytokine storm in pigs. Deletion of the MGF110-2L gene results in attenuation and induces protection against the parental virulent strain. These results support the induction of an alert immune state together with dampened systemic inflammation but preserved cytotoxic readiness in vaccinated animals. Altogether, our data shed light on the mechanisms underlying protection against ASFV and contribute to the development of new protective tools. Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/964 2026-01-01T00:00:00Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/963 Dostępność białka paszowego w Polsce w kontekście wykorzystania soi genetycznie zmodyfikowanej oraz surowców alternatywnych Mazur, Małgorzata; Sieradzki, Zbigniew; Król, Beata Biał ko jest jednym z naj waż niejszych składni ków pokar mo - wych w ży wieniu zwierząt gospo darskich, warun ku ją cym pra- wi dłowy wzrost, rozwój, pro duk cyj ność oraz zdrowot ność or- ga ni zmu. Jego podsta wo wą funkcją jest udział w bu do wie tka- nek oraz syntezie enzy mów, hormo nów i przeciw ciał. W prak - ty ce zootech nicznej odpo wied nie zaopa trze nie zwierząt w ten skład nik jest jednym z klu czowych elemen tów deter mi nu ją - cych efek tyw ność pro duk cji zwie rzę cej. Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/963 2026-01-01T00:00:00Z https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/962 GMO w łańcuchu żywnościowym Sieradzki, Zbigniew; Mazur, Małgorzata; Król, Beata Thu, 01 Jan 2026 00:00:00 GMT https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/962 2026-01-01T00:00:00Z