Repozytorium wiedzy PIWet - PIBRepozytorium cyfrowe DSpace zapisuje, przechowuje, indeksuje i udostępnia cyfrowe materiały.https://dspace.piwet.pulawy.pl:4432024-03-29T11:33:02Z2024-03-29T11:33:02ZCytokine inflammatory response in dairy cows with mastitis caused by Streptococcus agalactiaeBochniarz, MariolaHahaj-Siembida, AgataKrajewska-Wędzina, MonikaOsińska, MarcelinaTracz, AnnaTrościańczyk, AleksandraBrodzki, PiotrKrakowski, LeszekKosior-Korzecka, UrszulaNowakiewic, Anetahttps://dspace.piwet.pulawy.pl/xmlui/handle/123456789/6492024-03-28T11:04:59Z2024-01-01T00:00:00ZCytokine inflammatory response in dairy cows with mastitis caused by Streptococcus agalactiae
Bochniarz, Mariola; Hahaj-Siembida, Agata; Krajewska-Wędzina, Monika; Osińska, Marcelina; Tracz, Anna; Trościańczyk, Aleksandra; Brodzki, Piotr; Krakowski, Leszek; Kosior-Korzecka, Urszula; Nowakiewic, Aneta
IntroductionThe aim of the study was evaluation of the concentrations of interleukin (IL)-1β, IL-8, IL-12β and tumour necrosis factor alpha (TNF-α) in the serum and milk of cows with mastitis caused by Streptococcus agalactiae.Material and MethodsA total of 60 milk samples from diseased cows and 30 milk samples from healthy cows were included in the study. Blood and milk samples were taken from Holstein-Friesian cows from three herds (two in tie-stall and one in a free-stall housing system) in Lublin Province in Poland. The concentrations of cytokines in blood serum and quarter milk samples were determined by ELISA.ResultsThe levels of IL-1β, IL-8, IL-12β and TNF-α were significantly higher in the milk of cows suffering from mastitis caused by S. agalactiae compared to the milk of healthy cows (263.03 vs 55.36 pg/mL, 298.34 vs 131.82 pg/mL, 604.10 vs 139.17 pg/mL and 460.86 vs 78.82 pg/mL, respectively). In the group of sick cows, cytokine levels were significantly higher in milk than in serum (263.03 vs 55.25 pg/mL for IL-1β, 298.34 vs 164.22 pg/mL for IL-8, 604.10 vs 70.34 pg/mL for IL-12β and 460.86 vs 104.78 pg/mL for TNF-α).ConclusionThe results confirm the involvement of the entire bovine immune system to protect against the bacteria first locally in the udder. The response of the mammary gland to infection caused by S. agalactiae is rapid and already very strong at the beginning of the infection.
2024-01-01T00:00:00ZSerological characterisation of Lagovirus virus-like particles originating from native and mutated VP60 of rabbit haemorrhagic disease virus 2 and European brown hare syndrome virusKrejmer-Rąbalska, MartynaPelplińska, MartaSzewczyk, BogusławFitzner, Andrzejhttps://dspace.piwet.pulawy.pl/xmlui/handle/123456789/6482024-03-25T09:59:12Z2024-01-01T00:00:00ZSerological characterisation of Lagovirus virus-like particles originating from native and mutated VP60 of rabbit haemorrhagic disease virus 2 and European brown hare syndrome virus
Krejmer-Rąbalska, Martyna; Pelplińska, Marta; Szewczyk, Bogusław; Fitzner, Andrzej
Introduction: Since lagoviruses cannot be cultivated in vitro, using expression systems is an alternative and promising way of producing viral antigens for diagnostic purposes. It offers the possibility of their use as active immunogens for vaccine production. Materials and methods: We have produced virus-like particles (VLPs) in the baculovirus expression system in Sf9 insect cells based on wild-type and mutated variants of VP60 protein from the Polish strain of European brown hare syndrome virus (EBHSV) and wild-type and mutated versions of VP60 protein of Polish strain of rabbit haemorrhagic disease virus 2 (RHDV2). VLPs were purified with the use of sucrose gradient ultracentrifugation. Results: Protein production was confirmed by Western blot analysis using rabbit or hare sera and ELISA tests with different types of monoclonal antibodies. Hemagglutination properties of some VLPs have been evaluated as well. Electron microscopy of EBHSV-WT, RHDV2-WT and four produced VP60 variants revealed the formation of characteristic VLP structures. Conclusions: For the first time, we have obtained a mutated version of VLPs of RHDV2 with RGD motif in the VP60 protein sequence, which could be potentially used as a vehicle to deliver cargo to eukaryotic cells. We have also described VLPs based on VP60 protein from EBHSV and RHDV with three lysines substitutions in S and P2 domain. In addition, VLPs of EBHSV and RHDV2 could be potentially used as vaccine candidates.
2024-01-01T00:00:00ZMicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypesOstrycharz, EwaFitzner, AndrzejKęsy, AndrzejSiennicka, AldonaHukowska-Szematowicz, Beatahttps://dspace.piwet.pulawy.pl/xmlui/handle/123456789/6472024-03-22T12:43:15Z2024-01-01T00:00:00ZMicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypes
Ostrycharz, Ewa; Fitzner, Andrzej; Kęsy, Andrzej; Siennicka, Aldona; Hukowska-Szematowicz, Beata
MicroRNAs (miRs) are a group of small, 17–25 nucleotide, non-coding RNAthat regulate gene expression at the post-transcriptional level. To date, littleis known about the molecular signatures of regulatory interactions betweenmiRs and apoptosis and oxidative stress in viral diseases. Lagovirus europaeusis a virus that causes severe disease in rabbits (Oryctolagus cuniculus) calledRabbit Hemorrhagic Disease (RHD) and belongs to the Caliciviridae family,Lagovirus genus. Within Lagovirus europaeus associated with RHD, twogenotypes (GI.1 and GI.2) have been distinguished, and the GI.1 genotypeincludes four variants (GI.1a, GI.1b, GI.1c, and GI.1d). The study aimed toassess the expression of miRs and their target genes involved in apoptosisand oxidative stress, as well as their potential impact on the pathwaysduring Lagovirus europaeus—two genotypes (GI.1 and GI.2) infection ofdifferent virulences in four tissues (liver, lung, kidneys, and spleen). Theexpression of miRs and target genes related to apoptosis and oxidativestress was determined using quantitative real-time PCR (qPCR). In this study,we evaluated the expression of miR-21 (PTEN, PDCD4), miR-16b (Bcl-2,CXCL10), miR-34a (p53, SIRT1), and miRs—related to oxidative stress—miR-122 (Bach1) and miR-132 (Nfr-2). We also examined the biomarkers of bothprocesses (Bax, Bax/Bcl-2 ratio, Caspase-3, PARP) and HO-I as biomarkersof oxidative stress. Our report is the first to present the regulatory effectsof miRs on apoptosis and oxidative stress genes in rabbit infection withLagovirus europaeus—two genotypes (GI.1 and GI.2) in four tissues (liver,lungs, kidneys, and spleen). The regulatory effect of miRs indicates that,on the one hand, miRs can intensify apoptosis (miR-16b, miR-34a) in theexamined organs in response to a viral stimulus and, on the other hand, inhibit(miR-21), which in both cases may be a determinant of the pathogenesis ofRHD and tissue damage. Biomarkers of the Bax and Bax/Bcl-2 ratio promotemore intense apoptosis after infection with the Lagovirus europaeus GI.2genotype. Our findings demonstrate that miR-122 and miR-132 regulateoxidative stress in the pathogenesis of RHD, which is associated with tissuedamage. The HO-1 biomarker in the course of rabbit hemorrhagic disease indicates oxidative tissue damage. Our findings show that miR-21, miR-16b,and miR-34a regulate three apoptosis pathways. Meanwhile, miR-122 andmiR-132 are involved in two oxidative stress pathways.
2024-01-01T00:00:00ZThe European livestock resistomeMunk, P.Yang, D.Röder, T.Maier, L.Petersen, T. N.Duarte, A. S. R.Clausen, P. T. L.Brinch, C.Gompel, L. vanLuiken, R.Wagenaar, J. A.Schmitt, H.Heederik, D. J. J.Mevius, D. J.Smit, L. A. M.Graveland, H.Gonzalez-Zorn, B.Moyano, G.Sanders, P.Chauvin, C.Battisti, A.Dewulf, J.Wadepohl, K.Wasyl, D.Skarżyńska, M.Zając, M.Pękala-Safińska, A.Daskalov, H.Stärk, K.Aarestrup, F.M.https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/6462024-03-22T09:52:29Z2024-01-01T00:00:00ZThe European livestock resistome
Munk, P.; Yang, D.; Röder, T.; Maier, L.; Petersen, T. N.; Duarte, A. S. R.; Clausen, P. T. L.; Brinch, C.; Gompel, L. van; Luiken, R.; Wagenaar, J. A.; Schmitt, H.; Heederik, D. J. J.; Mevius, D. J.; Smit, L. A. M.; Graveland, H.; Gonzalez-Zorn, B.; Moyano, G.; Sanders, P.; Chauvin, C.; Battisti, A.; Dewulf, J.; Wadepohl, K.; Wasyl, D.; Skarżyńska, M.; Zając, M.; Pękala-Safińska, A.; Daskalov, H.; Stärk, K.; Aarestrup, F.M.
Understanding the occurrence, diversity, and drivers for antimicrobial resistance (AMR) is important to focus future control efforts. So far, almost all attempts to limit AMR in livestock have addressed antimicrobial consumption. We here performed an integrated analysis of the resistomes of five important farmed animal populations across Europe finding that the resistome and AMR levels are also shaped by factors related to bacterial diversity, as well as dispersal limitations. Thus, future studies and interventions aimed at reducing AMR should not only address antimicrobial usage but also consider other epidemiological and ecological factors.
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