artykuły z czasopism https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/6 2026-04-24T14:39:33Z 2026-04-24T14:39:33Z Niczyporuk, Samanta Jowita Kozdruń, Wojciech Grabarczyk, Piotr https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/941 2026-04-20T11:11:46Z 2026-01-01T00:00:00Z

West Nile Virus in Poland, what do we need to know about this infectious agent in 2025 — latest data Niczyporuk, Samanta Jowita; Kozdruń, Wojciech; Grabarczyk, Piotr West Nile virus (WNV) belongs to Flaviviridae family, genus Orthoflavivirus, species Orthoflavivirus nilense, and responsible for dangerous zoonosis, West Nile Fever (WNF). It is the most widespread arbovirus in the world. Is a neurotropic agent, transmitted by mosquitoes’ vectors mostly Culex genus, while the virus reservoir are wild and migratory birds various species. Virus transmission is also possible during blood transfusion and its components and organ transplantation, which is particularly dangerous for hematological and oncohematological patients. Encephalitis can occur in humans, and the virus has the ability to cross the blood-brain barrier. Global warming is undoubtedly a significant factor in the emergence of this pathogen in regions where it has not previously occurred.

2026-01-01T00:00:00Z Stępień-Pyśniak, Dagmara Tutaj, Krzysztof Niczyporuk, Jowita S. Sell, Bartosz Marek, Agnieszka Piekarska, Karolina Ognik, Katarzyna https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/940 2026-04-20T11:03:05Z 2026-01-01T00:00:00Z

Fatal poisoning of Old Polish ducks with Amanita muscaria Stępień-Pyśniak, Dagmara; Tutaj, Krzysztof; Niczyporuk, Jowita S.; Sell, Bartosz; Marek, Agnieszka; Piekarska, Karolina; Ognik, Katarzyna Background: Amanita muscaria (fly agaric) is a poisonous mushroom containing ibotenic acid (IBA) and muscimol (MUS), two neuroactive alkaloids capable of causing severe or fatal intoxication. While human poisoning is well documented, information on fatal intoxication in birds is limited. This report presents the first documented case of fatal poisoning of Old Polish ducks following ingestion of A. muscaria, confirmed by anatomopathological and toxicological analyses. Methods: Post-mortem specimens, including blood, heart, brain, kidney, liver, lung, pectoral and femoral muscles, and gastrointestinal contents, were subjected to LC–MS/MS analysis for the determination of IBA and MUS. Tissue samples were homogenized, extracted, derivatized, and quantified using multiple reaction monitoring. Mushroom caps and stems collected from the environment were analyzed using the same analytical approach. To exclude alternative toxicological etiologies, liver, muscle, kidney, and gastric contents were screened for rodenticides, pesticides, mycotoxins, and other chemical toxicants by LC–MS/MS. Viral infections were excluded by PCR and RT-PCR assays targeting DNA and RNA viruses commonly affecting waterfowl. In addition, comprehensive bacteriological, mycological, and parasitological examinations were conducted. Results: Post-mortem examination revealed lamellar mushroom fragments in the glandular stomach and congestion in the caeca and brain. Ibotenic acid (IBA) concentrations across tissues ranged from 4 to 1987 µg/kg, while muscimol (MUS) ranged from 2 to 66 µg/kg. In gastrointestinal contents, IBA and MUS concentrations ranged from 16.2 to 1110.5 µg/g and from 2 to 41.3 µg/g, respectively. Analysis of environmental mushroom material showed higher toxin levels in caps (871.7 µg/g IBA; 197.5 µg/g MUS) than in stems (206.6 µg/g IBA; 15.3 µg/g MUS). Screening of liver, muscle, kidney, and gastric contents excluded the presence of rodenticides, pesticides, mycotoxins, and other chemical toxicants, while PCR/RT-PCR and comprehensive bacteriological, mycological, and parasitological examinations ruled out viral, bacterial, fungal, and parasitic infections.

2026-01-01T00:00:00Z Biedermann, Anti Laurimäe, Teivi Anijalg, Liina Kamenetzky, Laura Soriano, Silvia V. Pierangeli, Nora Lazzarini, Lorena E. Umhang, Gérald Bold, Bolor Bayasgalan, Chimedtseren Karamon, Jacek Samorek-Pieróg, Małgorzata Simsek, Sami Celik, Figen Harandi, Majid F. Nasibi, Saeid Mehmood, Naunain Chihai, Oleg Casulli, Adriano Saarma, Urmas https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/939 2026-04-17T10:07:31Z 2026-01-01T00:00:00Z

Zoonotic Echinococcus granulosus sensu lato genotypes G6 and G7: new insights from the global mitogenome analysis Biedermann, Anti; Laurimäe, Teivi; Anijalg, Liina; Kamenetzky, Laura; Soriano, Silvia V.; Pierangeli, Nora; Lazzarini, Lorena E.; Umhang, Gérald; Bold, Bolor; Bayasgalan, Chimedtseren; Karamon, Jacek; Samorek-Pieróg, Małgorzata; Simsek, Sami; Celik, Figen; Harandi, Majid F.; Nasibi, Saeid; Mehmood, Naunain; Chihai, Oleg; Casulli, Adriano; Saarma, Urmas Cystic echinococcosis is a severe zoonotic disease caused by different species and genotypes belonging to the Echinococcus granulosus sensu lato (s.l.) complex. Among these, genotypes G6 and G7 are the second most common cause of human cystic echinococcosis. One of the very first steps towards understanding the epidemiology of G6 and G7 is to study their genetic and host diversity, population structure and phy- logenetic relationships. For this, we sequenced near-complete mitochondrial genomes (12,850– 12,856 bp) of 72 new G6 and G7 samples from eight countries and six host species, including humans. By adding 103 sequences from previous studies, the total dataset for further analyses comprised of 175 sequences from 20 countries and seven host species. This is the most comprehensive global mitogen- ome study of Echinococcus granulosus s.l. G6 and G7 to date. The results of this work revealed: (i) a new divergent haplogroup G6b from Mongolia; (ii) the subdivision of genotype G6 into two major hap- logroups: G6a (the nominal haplogroup) and G6b (the Mongolian haplogroup); (iii) highly divergent hap- lotypes of G6 and G7; (iv) the first molecularly confirmed findings of genotype G7 in camel; (v) genotype G7 in sheep – a rare species for G7; (vi) the importance of using long DNA sequences in phylogenetic analysis.

2026-01-01T00:00:00Z Bojarska, Katarzyna Kondratek, Katarzyna Leszczyńska, Aleksandra Sionek, Karolina Hatlauf, Jennifer Karamon, Jacek Samorek-Pieróg, Małgorzata Okarma, Henryk Śnieżko, Stanisław Didkowska, Anna Orłowska, Blanka Pyziel, Anna M. https://dspace.piwet.pulawy.pl/xmlui/handle/123456789/938 2026-04-17T09:53:47Z 2026-01-01T00:00:00Z

Golden jackals as hosts of zoonotic parasites: preliminary findings from southern Poland Bojarska, Katarzyna; Kondratek, Katarzyna; Leszczyńska, Aleksandra; Sionek, Karolina; Hatlauf, Jennifer; Karamon, Jacek; Samorek-Pieróg, Małgorzata; Okarma, Henryk; Śnieżko, Stanisław; Didkowska, Anna; Orłowska, Blanka; Pyziel, Anna M. Introduction: The golden jackal, Canis aureus, has been rapidly expanding its range across Europe, raising concerns regarding its impact on the health of wildlife, domestic animals and humans. The goal of this study was to examine the parasitological fauna of golden jackals that had recently colonised southern Poland. Material and Methods: The direct flotation method with centrifugation was used to search for parasite eggs, and a complex PCR and qPCR were run to detect the presence of tapeworms in nine faecal samples found by dogs and confirmed genetically, and in two samples taken from hunted individuals. The intestine contents of the hunted individuals were also examined using the sedimentation and counting technique. Camera traps were set to assess if the activity of golden jackals overlapped spatially with that of domestic animals. Results: Nine out of eleven faecal samples contained eggs of the Toxocara and Trichuris genera and the Capillariidae and Ancylostomatidae families. Mesocestoides litteratus DNA was detected in three faecal samples and Taenia serialis DNA in one sample collected from a hunted golden jackal. The sites frequented by domestic cats and dogs were also visited by golden jackals. Conclusion: Although golden jackals may be suspected of contributing to the transfer of some parasites to humans through domestic animals, the findings do not indicate that golden jackals represent a significant current or emerging threat to the health of wildlife or humans in southern Poland

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